Olfat Hammam^1*, Tarek Aboushousha¹, Ali El-Hindawi², Hosni Khairy², Heba Khalil¹, Amira Kamel¹, Maha Akl¹, Ahmed Abdel-Hady¹, Mona Magdy¹, Mohamed Badawy¹, Amr El Kholy¹, Khalid Al Osili¹, Nora Kamel³, Shady Anis², Tarek El Leithy¹

¹Theodor Bilharz Research Institute, Imbaba, Giza, Cairo, Egypt; ²Faculty of Medicine Cairo University, Cairo, Egypt; ³National Research Center5, El Doki, Giza, Egypt

BACKGROUND: Bladder cancer represents the fifth most common malignancy worldwide and a major cause of cancer-related morbidity and death. Incidence and mortality rates have remained relatively constant over the past four decades. Urothelial bladder cancers have identified multiple risk factors.

AIM: We aimed at evaluating the expression of the FGFR3 protein and gene amplification in the urothelial cells of neoplastic and non-neoplastic urothelial lesions of the urinary bladder, and correlation with tumour grade, stage and associated bilharziasis.

MATERIAL AND METHODS: One hundred and five different urinary bladder lesions were studied, including 15 cystitis cases (9 bilharzial and 6 non-bilharzial cystitides), 75 urothelial carcinoma cases (18 bilharzial associated and 57 non-bilharzial associated) and 15 squamous cell carcinoma associated with bilharziasis, beside 5 control cases. Data concerning age, sex, tumour grade, stage, and associated bilharziasis were obtained. Each case was studied for FGFR3 expression, and FISH technique was applied on forty malignant cases that show high protein expression.

RESULTS: The highest incidence of cystitis was in the fourth decade while of bladder cancer was in the seventh decade. Tumour grade was correlated significantly with tumour stage. FGFR3 correlates significantly with tumour grade, stage and with a bilharzial infestation. FGFR3 gene amplification was reported mainly in low grade and NNMBIC tumours.

Citation: Hammam O, Aboushousha T, El-Hindawi A, Khairy H, Khalil H, Kamel A, Akl M, Abdel-Hady A, Magdy M, Badawy M, El Kholy A, Al Osili K, Kamel N, Anis S, El Leithy T. Expression of FGFR3 Protein and Gene Amplification in Urinary Bladder Lesions in Relation to Schistosomiasis. Open Access Maced J Med Sci. https://doi.org/10.3889/oamjms.2017.048
Keywords: FGFR3; IHC; FISH; urothelial bladder lesions; cystitis; carcinoma.
*Correspondence: Professor Dr. Olfat Hammam. Pathology Department, Theodor Bilharz Research Institute, El-Nile Street,Warrak El-Hadar, Imbaba P.O. Box 30, Giza 12411, Egypt. Mobile number:202 01001815577. E-mail: totoali1@hotmail.com
Received: 23-Dec-2016; Revised: 18-Feb-2017; Accepted: 21-Feb-2017; Online first: 08-Apr-2017
Copyright: © 2017 Olfat Hammam, Tarek Aboushousha, Ali El-Hindawi, Hosni Khairy, Heba Khalil, Amira Kamel, Maha Akl, Ahmed Abdel-Hady, Mona Magdy, Mohamed Badawy, Amr El Kholy, Khalid Al Osili, Nora Kamel, Shady Anis, Tarek El Leithy. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
Funding: This work was financed by TBRI internal project No. 115T. Principle Investigator: Prof. Dr Olfat Hammam.
Competing Interests: The authors have declared that no competing interests exist.
 

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