Ahmed Atwa¹, Rehab Hegazy^2*, Nermeen Shaffie³, Neamat Yassin², Sanaa Kenawy⁴

¹Egyptian Russian University, Badr City, Egypt; ²Pharmacology Department, Medical Division, National Research Center, Giza, Egypt; ³Pathology Department, Medical Division, National Research Center, Giza, Egypt; ⁴Pharmacology and Toxicology Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt

BACKGROUND: Rhabdomyolysis (RM)-induced acute renal failure (ARF) accounts for about 10–40% of all cases of ARF.

AIM: The present study investigated the possible protective effect of two nitric oxides (NO)-releasing third generation β-blockers, carvedilol (Carv) and nebivolol (Nebi), against RM-mimicking glycerol (Gly)-induced ARF in rats.

MATERIAL AND METHODS: After 24 h dehydration, rats received a single dose of 50% Gly (8 ml/kg, im). They were treated with vehicle, Carv (2.5 mg/kg/day, po) or Nebi (10 mg/kg, po) for 3 successive days starting from an hour prior to Gly injection. Evaluation of blood pressure and locomotor activity was performed during the experiment. 72 h following Gly administration, total protein in the urine, serum levels of creatinine, blood urea nitrogen, sodium and potassium as well as the renal contents of malondialdehyde, reduced glutathione and NO were assessed, together with a histopathological examination of renal tissues.

RESULTS: Carv and Nebi attenuated Gly-induced renal dysfunction and histopathological alterations. They decreased the Gly-induced oxidative stress and increased renal NO concentration. Restoration of normal blood pressure and improvement of locomotor activity were also observed.

CONCLUSION: The results clearly demonstrate protective effects of Carv and Nebi against renal damage involved in RM-induced ARF and suggest a role of their antioxidant and NO-releasing properties.
 

Citation: Atwa A, Hegazy R, Shaffie N, Yassin N, Kenawy S. Protective Effects of Vasodilatory Βeta-Blockers Carvedilol and Nebivolol against Glycerol Model of Rhabdomyolysis-Induced Acute Renal Failure in Rats. Open Access Maced J Med Sci. http://dx.doi.org/10.3889/oamjms.2016.082
Keywords: Rhabdomyolysis; Acute renal failure; Carvedilol; Nebivolol; Glycerol; NO; Rat.
*Correspondence: Rehab Hegazy, PhD Pharmacology Department, Medical Division, National Research Centre, Bohoth st., 12622, Dokki, Giza, Egypt. Tel: +201001507676. E-mail: rehab_hegazy@hotmail.com
Received: 21-Jun-2016; Revised: 09-Jul-2016; Accepted: 10-Jul-2016; Online first: 01-Aug-2016
Copyright: © 2016 Ahmed Atwa, Rehab Hegazy, Nermeen Shaffie, Neamat Yassin, Sanaa Kenawy.   This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Competing Interests: The authors have declared that no competing interests exist.

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