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Open Access Macedonian Journal of Medical Sciences.

http://dx.doi.org/10.3889/oamjms.2015.082

eISSN: 1857-9655

Basic Science

 

 

Chemical Composition and Antifungal Activity of Ocimum basilicum L. Essential Oil


 

Neveen Helmy Abou El-Soud1*, Mohamed Deabes2, Lamia Abou El-Kassem3, Mona Khalil4

1Complementary Medicine Department, Medical Researches Division, National Research Center, El-Behouth Street, Dokki, 12311 Cairo, Egypt; 2Food Toxicology and Contaminants Department, National Research Center, El-Behouth Street, Dokki, 12311 Cairo, Egypt; 3Pharmacognosy Department - National Research Center- El-Behouth Street,Dokki, 12311 Cairo, Egypt; 4Biochemistry Department – Gizan University - K.S.A., Cairo, Egypt

 

Abstract

 

 

BACKGROUND: The leaves of Ocimum basilicum L. (basil) are used in traditional cuisine as spices; its essential oil has found a wide application in perfumery, dental products as well as antifungal agents.

AIM: To assess the chemical composition as well as the in vitro antifungal activity of O. basilicum L. essential oil against Aspergillus flavus fungal growth and aflatoxin B1 production.

MATERIAL AND METHODS: The essential oil of O. basilicum was obtained by hydrodistillation and analysed using gas chromatography (GC) and GC coupled with mass spectrometry (GC/MS). The essential oil was tested for its effects on Aspergillus flavus (A. flavus) mycelial growth and aflatoxin B1 production in Yeast Extract Sucrose (YES) growth media. Aflatoxin B1 production was determined by high performance liquid chromatography (HPLC).

RESULTS: Nineteen compounds, representing 96.7% of the total oil were identified. The main components were as follows: linalool (48.4%), 1,8-cineol (12.2%), eugenol (6.6%), methyl cinnamate (6.2%), α-cubebene (5.7%), caryophyllene (2.5%), β-ocimene (2.1%) and α-farnesene (2.0%).The tested oil showed significant antifungal activity that was dependent on the used oil concentration. The complete inhibition of A. flavus growth was observed at 1000 ppm oil concentration, while marked inhibition of aflatoxin B1 production was observed at all oil concentrations tested (500, 750 and 1000 ppm).

CONCLUSION: These results confirm the antifungal activities of O. basilicum L. oil and its potential use to cure mycotic infections and act as pharmaceutical preservative against A. flavus growth and aflatoxin B1 production.

..................

Citation: Abou El-Soud NH, Deabes M, El-Kassem LA, Khalil M. Chemical Composition and Antifungal Activity of Ocimum basilicum L. Essential Oil. OA Maced J Med Sci. http://dx.doi.org/10.3889/oamjms.2015.082
Key words: Ocimum basilicum L. essential oil; chemical composition; antifungal activity; aflatoxins B1; Aspergillus flavus.
*Correspondence: Prof. Dr. Neveen Helmy Abou El-Soud, Complementary Medicine Department, Medical Researches Division, National Research Center, El-Behouth Street,Dokki, 12311 Cairo, Egypt. Tel: +02-01224359509, E-mail: neveenster@gmail.com
Received: 09-jun-2015; Revised: 21-Jun-2015; Accepted: 26-Jun-2015; Online first: 10-Jul-2015
Copyright: © 2015 Neveen Helmy Abou El-Soud, Mohamed Deabes, Lamia Abou El-Kassem, Mona Khalil. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Competing Interests: The authors have declared that no competing interests exist.
 

 

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- Deabes M
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- Khalil M


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