Open Access Macedonian Journal of Medical Sciences.
http://dx.doi.org/10.3889/oamjms.2015.082
eISSN: 1857-9655
Basic Science
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Chemical Composition and Antifungal Activity of Ocimum basilicum L.
Essential Oil
Neveen Helmy Abou El-Soud1*, Mohamed Deabes2,
Lamia Abou El-Kassem3, Mona Khalil4
1Complementary Medicine Department, Medical Researches Division,
National Research Center, El-Behouth Street, Dokki, 12311 Cairo, Egypt;
2Food Toxicology and Contaminants Department, National Research Center,
El-Behouth Street, Dokki, 12311 Cairo, Egypt; 3Pharmacognosy
Department - National Research Center- El-Behouth Street,Dokki, 12311 Cairo,
Egypt; 4Biochemistry Department – Gizan University - K.S.A.,
Cairo, Egypt
BACKGROUND:
The leaves of
Ocimum basilicum
L. (basil) are used in traditional cuisine as
spices; its essential oil has found a wide
application in perfumery, dental products as well as antifungal agents.
AIM:
To assess the chemical composition as well as the in vitro antifungal
activity of O. basilicum L. essential oil against
Aspergillus flavus
fungal growth and aflatoxin B1 production.
MATERIAL AND
METHODS :
The essential
oil of O.
basilicum
was
obtained by
hydrodistillation
and analysed using gas chromatography (GC) and GC coupled with mass
spectrometry (GC/MS). The essential oil was tested for its effects on
Aspergillus flavus
(A. flavus) mycelial growth and aflatoxin B1 production in Yeast
Extract Sucrose (YES) growth media. Aflatoxin B1 production was determined
by high performance liquid chromatography (HPLC).
RESULTS:
Nineteen
compounds, representing 96.7% of the total oil were identified. The main
components were as follows: linalool (48.4%), 1,8-cineol (12.2%), eugenol
(6.6%), methyl cinnamate (6.2%), α-cubebene (5.7%), caryophyllene (2.5%), β-ocimene
(2.1%) and α-farnesene (2.0%).The
tested oil showed significant antifungal activity that was dependent on the
used oil concentration. The complete inhibition of A. flavus growth
was observed at 1000 ppm oil concentration, while marked inhibition of
aflatoxin B1 production was observed at all oil concentrations tested
(500, 750 and 1000 ppm).
CONCLUSION:
These
results confirm the antifungal activities of O. basilicum L. oil and
its potential use to cure mycotic infections and act as pharmaceutical
preservative against A. flavus
growth and aflatoxin B1 production.
..................
Citation: Abou El-Soud NH, Deabes M, El-Kassem
LA, Khalil M. Chemical Composition and Antifungal Activity of Ocimum
basilicum L. Essential Oil. OA Maced J Med Sci. http://dx.doi.org/10.3889/oamjms.2015.082
Key words: Ocimum basilicum L. essential oil; chemical
composition; antifungal activity; aflatoxins B1; Aspergillus flavus.
*Correspondence: Prof. Dr. Neveen Helmy Abou El-Soud, Complementary
Medicine Department, Medical Researches Division, National Research Center,
El-Behouth Street,Dokki, 12311 Cairo, Egypt. Tel: +02-01224359509, E-mail:
neveenster@gmail.com
Received: 09-jun-2015; Revised: 21-Jun-2015; Accepted: 26-Jun-2015; Online
first: 10-Jul-2015
Copyright: © 2015 Neveen Helmy Abou El-Soud, Mohamed Deabes, Lamia
Abou El-Kassem, Mona Khalil.
This is an open access article distributed under the terms of the Creative
Commons Attribution License, which permits unrestricted use, distribution,
and reproduction in any medium, provided the original author and source are
credited.
Competing Interests: The authors have declared that no competing
interests exist.
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Abou El-Soud NH
-
Deabes M
-
El-Kassem LA
-
Khalil M
PubMed
- Abou El-Soud NH
- Deabes M
- El-Kassem LA
- Khalil M
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