Macedonian Journal of Medical Sciences. 2012 Mar
15;
5(1):67-71.
http://dx.doi.org/10.3889/MJMS.1957-5773.2012.0223
Basic Science
Allele Frequency of HLADQB1 Locus in Macedonian Population
Eli Djulejic1, 2, Aleksandar Senev1, Meri Kirijas1,
Slavica Hristomanova1, Aleksandar Petlichkovski1,
Dejan Trajkov1, Mirko Spiroski1
1Institute of Immunobiology and Human Genetics, Faculty of
Medicine, Ss. Cyril and Methodius University in Skopje, Skopje, Republic of
Macedonia; 2Quintiles, Belgrade, Republic of Serbia
Aim: The aim of this paper was to genotype HLA DQB1 locus in healthy
unrelated Macedonian population.
Material and Methods: Reverse Line Strip typing for HLA DQB1 locus
was performed on a population of 217 samples from healthy individuals. The
results were obtained as alleles, and as NMDP Codes. Alleles were genotyped
and expressed as high resolution with more than four digits with slash
between ambiguous alleles. NMDP Codes were expressed as four digits for
unambiguous results, and with combination of numbers and characters for the
certain ambiguous combination. We did not found any genotypic ambiguities in
HLA-DQB1.
Results: We have found 33 different alleles of HLA DQB1 in Macedonian
population, 12 of which were unambiguous with the frequency of 40.55%, and
21 were ambiguous with the frequency of 58.53%. The biggest frequency
(33.64%) was found for HLA DQB1*05 unambiguous groups, and for HLA DQB1*03
ambiguous groups (36.40%). The biggest allele frequency in Macedonian
population for HLA DQB1 was found for HLA DQB1*03NX (0301/01/01/02/09/13)
with 27.88%, followed with HLA DQB1*0502 with 13.82%, and HLA DQB1*02MN
(0201/02/03) with 10.37%.
Conclusion: Allele frequency of HLA DQB1 in Macedonian population is
similar with that found in other European populations and can be used for
anthropology and disease association studies.
..................
Citation: Djulejic E, Senev A, Kirijas M, Hristomanova S,
Petlichkovski A, Trajkov D, Spiroski M. Allele Frequency of HLADQB1 Locus
in Macedonian Population. Maced J Med Sci. 2012 Mar 15; 5(1):67-71.
http://dx.doi.org/10.3889/MJMS.1957-5773.2012.0223.
Key words: HLA DQB1; Reverse Line Strip typing; alleles; NMDP Codes;
Macedonian population.
Correspondence: Prof. Dr. Mirko Spiroski. Institute of Immunobiology
and Human Genetics, Faculty of Medicine, Ss. Cyril and Methodius University
in Skopje, 50 Divizija No 16, 1109 Skopje, PO Box 60, Republic of Macedonia.
URL: http://www.iibhg.ukim.edu.mk E-mail: mspiroski@yahoo.com
Received: 17-Dec-2011; Revised: 09-Jan-2012; Accepted: 18-Feb-2012; Online
first: 22-Feb-2012
Copyright: © 2012 Djulejic E. This is an open access article
distributed under the terms of the Creative Commons Attribution License,
which permits unrestricted use, distribution, and reproduction in any
medium, provided the original author and source are credited.
Competing Interests: The authors have declared that no competing
interests exist.
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Djulejic E
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Senev A
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Kirijas M
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Hristomanova S
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Petlichkovski A
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Trajkov D
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Spiroski M
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Djulejic E
- Senev A
- Kirijas M
- Hristomanova S
- Petlichkovski A
- Trajkov D
- Spiroski M
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