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Macedonian Journal of Medical Sciences. 2012 Mar 15; 5(1):67-71.

http://dx.doi.org/10.3889/MJMS.1957-5773.2012.0223

Basic Science

 

Allele Frequency of HLA­DQB1 Locus in Macedonian Population
 

Eli Djulejic1, 2, Aleksandar Senev1, Meri Kirijas1, Slavica Hristomanova1, Aleksandar Petlichkovski1, Dejan Trajkov1, Mirko Spiroski1
 

1Institute of Immunobiology and Human Genetics, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, Skopje, Republic of Macedonia; 2Quintiles, Belgrade, Republic of Serbia

 

Abstract

 

 

Aim: The aim of this paper was to genotype HLA DQB1 locus in healthy unrelated Macedonian population.

Material and Methods: Reverse Line Strip typing for HLA DQB1 locus was performed on a population of 217 samples from healthy individuals. The results were obtained as alleles, and as NMDP Codes. Alleles were genotyped and expressed as high resolution with more than four digits with slash between ambiguous alleles. NMDP Codes were expressed as four digits for unambiguous results, and with combination of numbers and characters for the certain ambiguous combination. We did not found any genotypic ambiguities in HLA-DQB1.

Results: We have found 33 different alleles of HLA DQB1 in Macedonian population, 12 of which were unambiguous with the frequency of 40.55%, and 21 were ambiguous with the frequency of 58.53%. The biggest frequency (33.64%) was found for HLA DQB1*05 unambiguous groups, and for HLA DQB1*03 ambiguous groups (36.40%). The biggest allele frequency in Macedonian population for HLA DQB1 was found for HLA DQB1*03NX (0301/01/01/02/09/13) with 27.88%, followed with HLA DQB1*0502 with 13.82%, and HLA DQB1*02MN (0201/02/03) with 10.37%.

Conclusion: Allele frequency of HLA DQB1 in Macedonian population is similar with that found in other European populations and can be used for anthropology and disease association studies.

..................

Citation: Djulejic E, Senev A, Kirijas M, Hristomanova S, Petlichkovski A, Trajkov D, Spiroski M. Allele Frequency of HLA­DQB1 Locus in Macedonian Population. Maced J Med Sci. 2012 Mar 15; 5(1):67-71. http://dx.doi.org/10.3889/MJMS.1957-5773.2012.0223.
Key words: HLA DQB1; Reverse Line Strip typing; alleles; NMDP Codes; Macedonian population.
Correspondence: Prof. Dr. Mirko Spiroski. Institute of Immunobiology and Human Genetics, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, 50 Divizija No 16, 1109 Skopje, PO Box 60, Republic of Macedonia. URL: http://www.iibhg.ukim.edu.mk E-mail: mspiroski@yahoo.com
Received: 17-Dec-2011; Revised: 09-Jan-2012; Accepted: 18-Feb-2012; Online first: 22-Feb-2012
Copyright: © 2012 Djulejic E. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Competing Interests: The authors have declared that no competing interests exist.

 

 

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- Djulejic E
- Senev A
- Kirijas M
- Hristomanova S
- Petlichkovski A
- Trajkov D
- Spiroski M

PubMed

- Djulejic E
- Senev A
- Kirijas M
- Hristomanova S
- Petlichkovski A
- Trajkov D
- Spiroski M

 


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