Macedonian Journal of Medical Sciences. 2012 Mar
15;
5(1):62-66.
http://dx.doi.org/10.3889/MJMS.1957-5773.2012.0221
Basic Science
Influence of Hyperthermic Environmental Temperature on Adenosine Deaminase Activity in Serum and Lymphoid Organs of Wistar Rats
Slavica Hristomanova1, Dejan Trajkov1, Suzana
Dinevska-Kjovkarovska2, Aleksandar Petlichkovski1,
Slavcho Mitev2, Mirko Spiroski1
1Institute of Immunobiology and Human Genetics, Faculty of
Medicine, Ss. Cyril and Methodius University in Skopje, Skopje, Republic of
Macedonia; 2Departement of Physiology and Biochemistry, Faculty
of Natural Sciences, Ss. Cyril and Methodius University in Skopje, Skopje,
Republic of Macedonia
Aim: To analyze the influence of the hyperthermic environmental
temperature (35 ± 1°C) on the adenosine deaminase activity (ADA) in tissues
rich with lymphoid cells in Wistar rats.
Material and Methods: The activity of ADA was examined in thymus,
spleen, Payer’s patches, mesenteric lymph nodes and serum. The experimental
animals (n = 11 male rats) were exposed on hyperthermic environment (35 ±
1°C) and relative air humidity of 20 - 30% within a period of 30 days. The
control group of animals (n=10) were kept on room temperature (18 - 22°C).
Results: The body weight was decreased, spleen mass, and the thymus
mass were reduced. Activity of ADA in the thymus was significantly increased
from 10.58 ± 1.57 U/g in the control group of rats to 13.38 ± 2.7 U/g in
rats exposed on 35 ± 1°C (p<0.01), and in the spleen it raised from 5.17 ±
3.29 E/g to 7.8 ± 1.97 U/g (p<0.05). The ADA activity in the other lymphoid
organs and serum showed no statistically significant changes between the two
groups of examined rats.
Conclusions: Influence of hyperthermic environment (35 ± 1°C, 30
days) provokes decrease in body weight, spleen and thymus relative mass,
increase of the enzyme activity of ADA in thymus and spleen of rats, but
there are no changes in the other lymphoid organs and tissues.
..................
Citation: Hristomanova S, Trajkov D, Dinevska-Kjovkarovska S,
Petlichkovski A, Mitev S, Spiroski M. Influence of Hyperthermic
Environmental Temperature on Adenosine Deaminase Activity in Serum and
Lymphoid Organs of Wistar Rats. Maced J Med Sci. 2012 Mar 15; 5(1):62-66.
http://dx.doi.org/10.3889/MJMS.1957-5773.2012.0221.
Key words: Adenosine deaminase (ADA); thymus; spleen; acclimation;
Wistar rats.
Correspondence: Prof. Dr. Mirko Spiroski. Institute of Immunobiology
and Human Genetics, Faculty of Medicine, Ss. Cyril and Methodius University
in Skopje, 50 Divizija No 16, 1109 Skopje, PO Box 60, Republic of Macedonia.
URL: http://www.iibhg.ukim.edu.mk E-mail: mspiroski@yahoo.com
Received: 15-Dec-2011; Revised: 05-Jan-2012; Accepted: 14-Feb-2012; Online
first: 16-Feb-2012
Copyright: © 2012 Hristomanova S. This is an open access article
distributed under the terms of the Creative Commons Attribution License,
which permits unrestricted use, distribution, and reproduction in any
medium, provided the original author and source are credited.
Competing Interests: The authors have declared that no competing
interests exist.
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Hristomanova S
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Trajkov D
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Dinevska-Kjovkarovska S
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Petlichkovski A
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Mitev S
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Spiroski M
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Hristomanova S
- Trajkov D
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- Petlichkovski A
- Mitev S
- Spiroski M
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