Adejoke Y Onaolapo¹, Olakunle J Onaolapo²
 

¹Department of Human Anatomy, Ladoke Akintola University of Technology, Ogbomoso, Oyo state, Nigeria; ²Department of Pharmacology and Therapeutics, Ladoke Akintola University of Technology, Ogbomoso, Oyo state, Nigeria

Aim: Hepatoprotective effects of Ethanolic extract Ocimum gratissimum leaves (O. gratissimum) in streptozotocin-induced diabetic rats was studied.

Material and Methods: Thirty-six adult rats were assigned into six groups (A, B, C, D, E and F) of six rats each. Group A was the control, while diabetes was induced in groups B, C, D, E and F. Fasting blood glucose was determined and rats with blood glucose concentration >18.0 mmol/L were considered diabetic. Group A (non diabetic control) and F (diabetic control) received normal saline orally, group B received a Metformin at 25 mg/kg while groups C, D and E had O. gratissimum at 400, 600 and 800 mg/kg. Treatment was daily for a period of 28 days for all groups. Fasting blood glucose was monitored weekly, serum levels of Alanine transaminase (ALT) and Aspartate transaminase (AST) were measured on day twenty eight. Animals were sacrificed, liver sections processed for histological study. Statistical analysis was carried out using a one way ANOVA followed by a post-hoc test.

Results: Results showed reduction in blood sugar and increase in biochemical and histologic markers of hepatic injury.

Conclusion: Ethanolic extract of O. gratissimum causes dose dependent hepatic injury despite its glucose lowering ability.

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Citation: Onaolapo AY, Onaolapo OJ. Ocimum Gratissimum Linn Causes Dose Dependent Hepatotoxicity in Streptozotocin-Induced Diabetic Wistar Rats. Maced J Med Sci. 2012 Mar 15; 5(1):17-25. http://dx.doi.org/10.3889/MJMS.1957-5773.2011.0206.
Key words: Ocimum gratissimum; Streptozotocin; Diabetes Mellitus; Hepatotoxicity.
Correspondence: Adejoke Y. Onaolapo. Department of Human Anatomy, Ladoke Akintola University of Technology, Ogbomoso, Oyo state, Nigeria. Phone: 2348062240434 E-mail: olakunleonaolapo@yahoo.co.uk, adegbayibiy@yahoo.com
Received: 09-Oct-2011; Revised: 14-Nov-2011; Accepted: 15-Nov-2011; Online first: 05-Dec-2011
Copyright: © 2011 Onaolapo AY. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Competing Interests: The author have declared that no competing interests exist.
 

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