Macedonian Journal of Medical Sciences. 2011 Mar 15;
4(1):37-43.
doi:10.3889/MJMS.1857-5773.2011.0147
Basic Science
Identification and Cementoblastic / Osteoblastic
Differentiation of Postnatal Stem Cells from Human Periodontal Ligament
Maha Abd El Fattah1,2, Gang Ding1, Fulan Wei1,
Chunmei Zhang1, Eman Aboul Ezz2, Songlin Wang1,3
1Salivary Gland Disease Center and the Molecular Laboratory for
Gene Therapy and Tooth Regeneration, Capital Medical University School of
Stomatology. Tian Tan Xi Li No.4, Beijing 100050, China; 2Oro-dental
genetics department, National Research Center, Cairo, Egypt; 3Department
of Biochemistry and Molecular Biology, Capital Medical University School of
Basic Medical Sciences, You An Men Wai No.10, Beijing 100069, China
Background.
Periodontal diseases that lead to the destruction of periodontal tissues,
including periodontal ligament (PDL), cementum, and bone, are a major cause
of tooth loss in adults and are a substantial public health burden
worldwide. PDL is a specialized connective tissue that connects cementum and
alveolar bone to maintain and support teeth in situ and preserve tissue
homoeostasis. In this study we aimed to isolate, identify periodontal
ligament stem cells and their osteoblastic/ cementoblastic differentiation.
Methods. Periodontal ligament tissue was obtained from human impacted
third molars (n=5) from different individuals from the oral surgery
department, Department of Oral and Maxillofacial Surgery, Capital Medical
University School of Stomatology (Beijing, China) following which a colony
forming unit – fibroblast assay, identification of periodontal ligament stem
cells (PDLSCs; STRO-1 + & CD146+) by using immunocytofluorescence and
isolation of PDLSCs (STRO-1+) by using flow cytometry and cementoblastic/osteoblastic
exvivo induction were performed.
Results. Mesenchymal stem cells were identified in the periodontal
ligament derived by their capacity to form adherent clonogenic cell
clusters. Ex-vivo expanded periodontal ligament stem cells were found to
express the mesenchymal stem cell markers STRO-1 and CD146. Flow cytometric
study showed that a total of 24.53% of periodontal ligament cell population
stained positive for the STRO-1 antibody and of that population 1.14% were
strongly positive.
Conclusions. The finding of this study indicated that some PDL cells
possess crucial stem cells properties, such as self renewal and express the
mesenchymal stem cell markers (STRO-1 and CD 146) on their cell surface and
small round alizarin red-positive nodules formed in the PDLSC cultures after
4 weeks of induction, indicating calcium accumulation in vitro. Thus, PDL
cells can be used for periodontal regenerative procedures.
..................
Citation: El Fattah MA, Ding G, Wei F,
Zhang C, Ezz EA, Wang S. Identification and Cementoblastic / Osteoblastic
Differentiation of Postnatal Stem Cells from Human Periodontal Ligament.
Maced J Med Sci. 2011 Mar 15; 4(1):37-43.
doi.10.3889/MJMS.1957-5773.2011.0147.
Key words: Stem Cells; Periodontal Ligament; Differentiation;
Cementoblast; Osteoblast.
Correspondence: Prof. Songlin Wang. Salivary Gland Disease Center and
the Molecular Laboratory for Gene Therapy and Tooth Regeneration, Capital
Medical University School of Stomatology. Tian Tan Xi Li No.4, Beijing
100050, PR China. Tel. /Fax +86 10 6706 2012, E-Mail: slwang@ccmu.edu.cn
Received: 05-Jun-2010; Revised: 30-Aug-2010; Accepted: 28-Sep-2010; Online
first: 28-Jan-2011
Copyright: © 2011 El Fattah MA. This is an open-access article
distributed under the terms of the Creative Commons Attribution License,
which permits unrestricted use, distribution, and reproduction in any
medium, provided the original author and source are credited.
Competing Interests: The author have declared that no competing
interests exist. |
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