Macedonian Journal of Medical Sciences
[International Peer Reviewed]

 

About MJMS

Our policies

MJMS Online

For contributors

Services

Why publish in MJMS?
Editors
Boards
Indexing
Membership
Evaluation

Editorial & publishing policies
Competing interests policy 
Open access
Open access license

Publication fee
Reviewer guidelines

Online first
Current issue
Journal archive

Online first fact sheet
Free Registration
Contact

Guidelines  [pdf]
ICMJE Disclosure Form
Online submission
Help for authors
Cover letter
Reviewers of MJMS

Transliteration
Subscriptions

Advertising
Reprints and permissions
Resources

 

Macedonian Journal of Medical Sciences. 2011 Mar 15; 4(1):37-43.

doi:10.3889/MJMS.1857-5773.2011.0147

Basic Science

 

Identification and Cementoblastic / Osteoblastic Differentiation of Postnatal Stem Cells from Human Periodontal Ligament
 

Maha Abd El Fattah1,2, Gang Ding1, Fulan Wei1, Chunmei Zhang1, Eman Aboul Ezz2, Songlin Wang1,3 

1Salivary Gland Disease Center and the Molecular Laboratory for Gene Therapy and Tooth Regeneration, Capital Medical University School of Stomatology. Tian Tan Xi Li No.4, Beijing 100050, China; 2Oro-dental genetics department, National Research Center, Cairo, Egypt; 3Department of Biochemistry and Molecular Biology, Capital Medical University School of Basic Medical Sciences, You An Men Wai No.10, Beijing 100069, China

 

Abstract

 

 

Background. Periodontal diseases that lead to the destruction of periodontal tissues, including periodontal ligament (PDL), cementum, and bone, are a major cause of tooth loss in adults and are a substantial public health burden worldwide. PDL is a specialized connective tissue that connects cementum and alveolar bone to maintain and support teeth in situ and preserve tissue homoeostasis. In this study we aimed to isolate, identify periodontal ligament stem cells and their osteoblastic/ cementoblastic differentiation.

Methods. Periodontal ligament tissue was obtained from human impacted third molars (n=5) from different individuals from the oral surgery department, Department of Oral and Maxillofacial Surgery, Capital Medical University School of Stomatology (Beijing, China) following which a colony forming unit – fibroblast assay, identification of periodontal ligament stem cells (PDLSCs; STRO-1 + & CD146+) by using immunocytofluorescence and isolation of PDLSCs (STRO-1+) by using flow cytometry and cementoblastic/osteoblastic exvivo induction were performed.

Results. Mesenchymal stem cells were identified in the periodontal ligament derived by their capacity to form adherent clonogenic cell clusters. Ex-vivo expanded periodontal ligament stem cells were found to express the mesenchymal stem cell markers STRO-1 and CD146. Flow cytometric study showed that a total of 24.53% of periodontal ligament cell population stained positive for the STRO-1 antibody and of that population 1.14% were strongly positive.

Conclusions. The finding of this study indicated that some PDL cells possess crucial stem cells properties, such as self renewal and express the mesenchymal stem cell markers (STRO-1 and CD 146) on their cell surface and small round alizarin red-positive nodules formed in the PDLSC cultures after 4 weeks of induction, indicating calcium accumulation in vitro. Thus, PDL cells can be used for periodontal regenerative procedures.

 

..................

Citation: El Fattah MA, Ding G, Wei F, Zhang C, Ezz EA, Wang S. Identification and Cementoblastic / Osteoblastic Differentiation of Postnatal Stem Cells from Human Periodontal Ligament. Maced J Med Sci. 2011 Mar 15; 4(1):37-43. doi.10.3889/MJMS.1957-5773.2011.0147.
Key words: Stem Cells; Periodontal Ligament; Differentiation; Cementoblast; Osteoblast.
Correspondence: Prof. Songlin Wang. Salivary Gland Disease Center and the Molecular Laboratory for Gene Therapy and Tooth Regeneration, Capital Medical University School of Stomatology. Tian Tan Xi Li No.4, Beijing 100050, PR China. Tel. /Fax +86 10 6706 2012, E-Mail: slwang@ccmu.edu.cn
Received: 05-Jun-2010; Revised: 30-Aug-2010; Accepted: 28-Sep-2010; Online first: 28-Jan-2011
Copyright: © 2011 El Fattah MA. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Competing Interests: The author have declared that no competing interests exist.

 

< Previous | Next Article >

Table of contents

 

This Article (free)

Abstract

Macedonian Abstract

Full text (html)

Full text (pdf)

Full text OnlineFirst (pdf)

Citations

- DOAJ

- Index Copernicus

- Socol@ar

Google Scholar

- El Fattah MA

- Ding G

- Wei F

- Zhang C

- Ezz EA

- Wang S

PubMed

- El Fattah MA
- Ding G
- Wei F
- Zhang C
- Ezz EA
- Wang S
 


Publication of the MJMS is supported by the Macedonian Ministry of Education and Sciences. Publisher: Institute of Immunobiology and Human GeneticsSkopje, Republic of Macedonia.

This journal is a member of and subscribes to the principles of the Committee on Publication Ethics.

MJMS Print (ISSN 1857-5749) is an international peer-reviewed, Open Access journal published four times per year. MJMS Online (ISSN 1857-5773) offers free access to all articles.


Creative Commons Attribution LicenseAll site content, except where otherwise noted, is licensed under a Creative Commons Attribution License.