Macedonian Journal of Medical Sciences
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Abstract                                                                         [Full-Text PDF] [Macedonian Abstract] [OnlineFirst Full-Text PDF]

 

Macedonian Journal of Medical Sciences. 2008 Dec 15; 1(2):59-61.

doi:10.3889/MJMS.1857-5773.2008.0008

Scientific Project

 

Ambiguities Resolution of HLA Genotypes in Macedonian Population

Mirko Spiroski, Aleksandar Petlichkovski, Ana Strezova

Institute of Immunobiology and Human Genetics, Faculty of Medicine, University “Ss Kiril and Metodij”, Skopje, Republic of Macedonia
 

A total of 850 persons from the Republic of Macedonia, divided in the four groups, were DNA-HLA genotyped: 1. Donors of the Macedonian Donor Registry (220); 2. Patients for bone marrow transplantation and related potential donors (350); 3. Patients with rheumatoid arthritis, tuberculosis, cardiomyopaties and other diseases (150); and 4. Ambiguity resolution of the genotypes (130). Class I HLA genes (A, C, B, and DQB1) were typed by Reverse Line Strip Typing (RLS); Class II HLA gene (DRB1) were typed by Sequence Based Typing (SBT). The main results of the project are: a) Creation and legislation of Macedonian Donor Registry, and Macedonian Human DNA Bank; b) HLA typing of unrelated healthy persons for class I HLA-A, -C, and –B, and class II HLA-DRB1; c) HLA genotyping of blood related samples for Bone Marrow Transplantation; d) Association analysis of HLA alleles with Rheumatoid Arthritis (HLA-DRB1, and HLA-B27), and with Pulmonary Tuberculosis (HLA-DQB1); e) Evaluation of allele and genotype ambiguities for HLA class I, and HLA class II, as well as resolution of HLA class I and class II ambiguities; f) Introduction and establishment of sequence specific primers (SSP) method for resolving HLA class II ambiguities in patients with Rheumatoid Arthritis.

 

Key words: HLA alleles; HLA haplotypes; ambiguities resolution; Macedonian Donor Registry.

 


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